Senior Scientist - In vitro cardiac disease modelling

Are you a curious and experienced scientist in the field of stem cells and gene editing? Would you like to contribute to the development of novel therapies? If yes, AstraZeneca might have the next challenge for you!

A thrilling opportunity have arisen for you to join us in the Applied Stem Cell Sciences group in Translational Genomics, Discovery Sciences at AstraZeneca in Gothenburg, Sweden. We are looking for our next peer to come work with us as a bench-based Senior Scientist - to be a key player in developing therapeutics to change patients' lives.

Join a place built on innovation and creativity. Where different views and perspectives are welcomed and valued.

What you will do:
You will work alongside our stem cell scientists, disease area specialists and genome editing group. Using your strong knowledge and experience in stem cell biology, cell model development and CRISPR technology you will have the opportunity to work on in vitro aspects of therapeutic genome editing projects. This includes culture, genome-engineering and differentiation of induced pluripotent stem cells (iPSCs) and genetic and functional validation of engineered lines.

The role is focused on laboratory-based activities. Your biggest impact will be through significant contributions to the practical delivery to projects in compliance with AstraZeneca corporate responsibility, policies and relevant safety standards. Additional key responsibilities:

* Culture and differentiation of human iPSC lines to cardiac and skeletal muscle.
* Perform molecular biology, biochemical, and cellular assays to evaluate the outcomes of gene editing experiments on genomic DNA, mRNA, protein, and functional levels.
* Culture of human primary cells and various mammalian cell lines.
* Collaborate within an exciting matrixed research team to facilitate the success of projects.

Essential in the role:

* PhD in Developmental Biology, Cell Biology, Gene Editing or a BSc/MSc degree with significant experience.
* Proven experience with PSC biology and directed-differentiation to cardiac and skeletal muscle.
* Demonstrable experience with cell line engineering using CRISPR/CAS9.
* Design, develop, and optimize in vitro transfection/transduction experiments using a variety of perturbagens, including lentivirus/AAV-mediated infection.
* Hands-on experience with the following assay platforms: RT-qPCR, ddPCR, Flow Cytometry, ELISA, Western blots, and cellular functional assays.
* Experience with the culture of multiple mammalian cell types including primary cells.

It is also essential to have good social and communication skills, be collaborative and a strong team player. You should be able to work independently, take own initiatives and have a positive, goal oriented and problem-solving attitude. Good organizational, data and documentation skills are required, and you should be comfortable in presenting your results. English language is required, both spoken and in writing.

Desirable in the role:

* Experience with NGS library prep, bioinformatic analysis, and cutting-edge molecular biology methods.
* Expertise with liquid handling automation technologies.
* Experience of setting up cell based pharmacological assays.

Why AstraZeneca in Gothenburg?
With more than 2,400 employees from over than 50 countries, our vibrant Gothenburg site is a truly inspiring place to work. Here, the history and future of scientific breakthrough come together. We believe that the diversity of our people is crucial to bringing new discoveries to life. Here we are forever pushing the boundaries as we feel comfortable spotting opportunities, making quick decisions and taking smart risks.

So what's next? If this sounds like the next job and place for you, welcome to apply today!

We look forward to get to know you better - please send your application to us as soon as possible, but no later than 12th June, 2022.

For more information about the position please contact hiring manager Bilada Bilican at bilada.bilican(at)astrazeneca.com.

Additional information:
Our Gothenburg site: https://www.astrazeneca.com/our-science/gothenburg.html
Life in Gothenburg: https://www.movetogothenburg.com/
Make a move to Global Gothenburg: https://www.youtube.com/watch?v=c-Iqbb4aw38
About BioPharmaceuticals R&D: https://www.astrazeneca.com/r-d.html