Thesis work: Tissue clearing and immunolabeling for 3D imaging

Job description
 Tssue clearing and immunolabeling of kidney, heart , and liver to enable 3D organ imaging

Thesis type: 30 credits
Project start: January 2023
Student location: On Site, Gothenburg Sweden

Do you have experience of and interest in working with histology? Would you like to develop tissue clearing protocols for whole organ imaging ? Then this Master thesis project at AstraZeneca might be of interest to you!

You will join Cell and Molecular Pharmacology (CMP) team that delivers key data within histology, imaging, biomarker, FACS and bioassay automation to Cardiovascular, Renal and Metabolism (CVRM) disease areas. Within the histology group we are at the scientific forefront of latest histology technique and image analyses. 



Responsibilities
State- of- Art tissue clearing combined with fluorescent labelling (tissue structures/compounds/antibodies/proteins/cells) and imaging can provide valuable visualization of 3D structure together with target distribution through whole organ. 

3D organ imaging is hampered due to low light penetration through the tissue. Major cause of low tissue transparency is light scattering caused by organ components such as lipids, pigments and collagen fibers. By using optical clearing agents (OCAs) these disturbing components are removed from the tissue, leading to improvement of light penetration and increasing tissue transparency and enabling whole organ imaging.

The  goal of thesis is to optimize clearing protocol to achieve tissue transparency and  multiplex fluorescent labelling in diverse mouse organs to enable 3D imaging 

As master student you will be a part of our histology team and learn about tissue fixation, processing, labelling, and imaging.

We look forward working with you!



Qualifications
Essential Requirements 
Well-organized and focused mindset
Strong oral and written communication skills. 

Desirable Requirements
Desire to learn and try new things. 


Application
Deadline 2022-11-04

About the company
 At AstraZeneca, all of our employees make a difference to patients’ lives every day. We operate in more than 100 countries around the world and are one of Sweden’s most important export companies. With more than 2,400 employees from 50 countries, the vibrant Gothenburg site helps to support the entire life-cycle of AstraZeneca medicines, from drug discovery and clinical trials, to global commercialisation and product maintenance. Gothenburg is one of AstraZeneca’s three strategic, global R&D centres, alongside Cambridge and Gaithersburg, and plays a central role in our mission to deliver life-changing medicines to patients.